Crystallization and preliminary X-ray analysis of Sau3AI C-terminal 232-419 amino acids fragment.
نویسندگان
چکیده
The C-terminal 232-419 amino acids fragment of endonuclease Sau3AI has been successfully expressed in Escherichia coli with 6 His at its N-terminal. After purification and crystallization, one completed 2.8 A data set was collected using a Rigaku R-AXIS IV++ diffractometer. The plate-like crystals belong to orthorhombic space group P2(1)2(1)2(1) with the cell dimension of a = 34.75, b = 76.82, c = 123.59A and contain one molecule per asymmetric unit.
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ورودعنوان ژورنال:
- Protein and peptide letters
دوره 13 6 شماره
صفحات -
تاریخ انتشار 2006